Denis Rousseau (Eds.)'s Optical Techniques in Biological Research PDF

By Denis Rousseau (Eds.)

ISBN-10: 0125993226

ISBN-13: 9780125993227

ISBN-10: 0323150217

ISBN-13: 9780323150217

Optical recommendations in organic Research

summary: Optical recommendations in organic examine

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We shall use this rather general formalism for a specific purpose shortly. It is also useful to recognize that a variable of position X(R) may be represented as a series of oscillating functions of position, for example, cos(R · k), where |k| = 2π/λ is the spatial equivalent of frequency. The variable may be represented as a function of either R or k, and the two representations may be interconverted via space Fourier transforms: X(k) = (2n)-1 ( XCR)é>*'R-*R

70) this time will be proportional to the square of the distance (l/K2) and inversely proportional to the diffusion coefficient, and thus proportional to (l/'DK2). To obtain the spectrum of the scattered light, we need only to perform a time Fourier transform [Eq. (52)] of Eq. (69). In relative units we may write the spectrum as T/ x ω) « -(ω-α# DK2/n + φΚ*Τ (71) Equation (71) describes a Lorentzian distribution whose half-width at halfheight is given (in angular frequency units) by DK2. The result of this derivation, stated in Eqs.

12 Diffusion coefficients extrapolated to zeroribonucleaseconcentration cor­ rected to the viscosity of water at 20 °C versus temperature. 2. The decrease in diffusion coefficient with increasing temperature reveals the unfolding transition from native (state A) to denatured (state B) protein. ) of anisotropic particles, and intramolecular motions, may complicate the data. These effects may in fact be of greater interest than a knowledge of the translational diffusion coefficient, and so considerable effort has been ap­ plied to the problem of utilizing QELS in these areas, as discussed in the remainder of this section.

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Optical Techniques in Biological Research by Denis Rousseau (Eds.)

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