By Uttam Garg, Catherine A. Hammett-Stabler
As mass spectrometric equipment now provide a degree of specificity and sensitivity unrealized through spectrophotometric- and immunoassay-based equipment, mass spectrometry has entered the medical laboratory the place it's getting used for a variety of functions. In Clinical functions of Mass Spectrometry: equipment and Protocols, professional researchers supply distinct step by step methods for the research of variety of analytes of scientific value. this flexible and expansive quantity covers mass spectrometry equipment for analytes together with various medications, hormones, and metabolic compounds spanning the disciplines of toxicology, healing drug tracking, endocrinology, and pediatric metabolism. Written within the hugely winning Methods in Molecular Biology™ sequence layout, chapters contain short introductions to the analytes, lists of the mandatory fabrics and reagents, conveniently reproducible analytical protocols, and distinct notes on troubleshooting and averting identified pitfalls.
Comprehensive and loyal, Clinical functions of Mass Spectrometry: tools and Protocols bargains its readers a wide range of necessary equipment for knowledgeable mass spectrometric labs which are seeking to introduce new analyses in addition to for these laboratories at present contemplating the addition of this inventive and very important technology.
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Extra resources for Clinical Applications of Mass Spectrometry: Methods and Protocols
Add 5 mL of ethyl acetate to each tube. 5. Cap all tubes with large Teflon disposable caps and place on block vortex for 10 min. 6. Centrifuge at 3000 Â g for 5 min. 7. Transfer the organic layer to a small test tube using a glass transfer pipette. 8. Add 100 mL of 1% (v/v) HCl in methanol to each tube. 9. Dry samples down under nitrogen. 10. Add 25 mL of ethyl acetate and 25 mL HFBA to each sample tube. 11. Cap with non-vented caps and place in the 75°C heat block for 30 min. 12. Dry down under nitrogen.
There are five categories of antidepressants based on function and/or structure. These are monoamine oxidase inhibitors (MAOI), cyclic antidepressants including tricyclic and tetracyclic compounds (TCA), selective serotonin reuptake inhibitors (SSRI), serotonin-norepinephrine reuptake inhibitors (SNRI), and atypical compounds. This method is designed to detect the presence of antidepressant drugs in blood/serum, urine, and tissue specimens using gas chromatography/mass spectrometry (GC/ MS) following liquid–liquid extraction (LLE) and identified by relative retention times and mass spectra.
2, Applied Biosystems, Foster City, CA). Q3) were previously determined empirically using aqueous solutions of individual amino acids (see Note 3). 10. Identification and quantitation are based on a single precursor–product ion pair and retention time. 2 (see Notes 4–6). 11. Chromatography is stable for 2 months or approximately 400 injections. 2 Chromatography and MS/MS parameters for each amino acid and deuterated internal standards. Absolute RT ¼ typical C8 retention time; Relative RT ¼ typical retention time relative to retention time of respective internal standard.
Clinical Applications of Mass Spectrometry: Methods and Protocols by Uttam Garg, Catherine A. Hammett-Stabler